Carbohydrate sensing with a metal-based indicator displacement assay.
نویسندگان
چکیده
A Pd-based indicator displacement assay for the fluorimetric sensing of carbohydrates has been developed. The assay allows the detection of saccharides in the low millimolar concentration range in water at neutral pH.
منابع مشابه
Pattern-based sensing of nucleotides in aqueous solution with a multicomponent indicator displacement assay.
A multicomponent indicator displacement assay (MIDA) based on an organometallic receptor and three dyes can be used for the identification and quantification of nucleotides in aqueous solution at neutral pH.
متن کاملSelective Dna Sensing Elements Integrated into Microfluidic Channels
This paper describes the development of a unique fluorescence based DNA diagnostic assay that does not require labeling of the target sequence prior to analysis. The assay uses polyacrylamide hydrogel plugs immobilized in microfluidic channels, and is based on the displacement of a short sacrificial fluorescent-tagged indicator oligomer by a longer untagged target sequence as it is electrophore...
متن کاملPyridinium-based symmetrical diamides as chemosensors in visual sensing of citrate through indicator displacement assay (IDA) and gel formation.
The design and synthesis of pyridinium-based symmetrical diamides 1 and 2 along with their anion binding studies through 'indicator-diplacement assay' are reported. Both the chemosensors effectively respond in in CH(3)CN-H(2)O (4 : 1 v/v) at pH = 6.3 for the selective naked-eye detection of citrate. Additionally, chemosensor 2 (c = 6.29 × 10(-3) M) forms a stable gel only with citrate in CH(3)C...
متن کاملPyridinium-based tripodal chemosensor in visual sensing of AMP in water by indicator displacement assay (IDA).
A simple pyridinium-based tripodal chemosensor, 1, effectively recognizes AMP over ATP and ADP through indicator displacement assay (IDA) technique in water at pH 6.4. The good recognition of 1 is due to the better accommodation of AMP at the core of 1 as well as functional interaction involving hydrogen bonding and charge-charge interaction. The sensor 1 also recognizes intracellular AMP.
متن کاملAssociative chemosensing by fluorescent macrocycle-dye complexes – a versatile enzyme assay platform beyond indicator displacement.
A label-free in situ method to monitor reactions in real time by using fluorescent supramolecular chemosensors based on cucurbit[8]uril is presented. It allows sensing of enzymatic activity, inhibitor and activator screening, and analyte detection with unprecedented versatility and high sensitivity.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Chemical communications
دوره 47 30 شماره
صفحات -
تاریخ انتشار 2011